The sanctuary extends from the mean high tide line in Maryland state waters across the Potomac River to the mean low tide in Virginia state waters. The tidal freshwater river that comprises the majority of the sanctuary supports species of ecological, economic, and cultural importance, while the shipwrecks of the Ghost Fleet serve as islands of life that provide structure and habitat. Adjacent to sanctuary waters, Maryland’s Mallows Bay Park hosts wetlands, meadows, and forests that have been identified as highly significant for biodiversity conservation, habitat connectivity, and rare species and wildlife habitat.